3RAP

The small G protein Rap2 in a non catalytic complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	41.010, 59.830, 68.440
Unit cell angles	90.00, 97.07, 90.00

Refinement procedure

Resolution	16.000 - 2.200
R-factor	0.211
Rwork	0.211
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RAP2A-GTP HEXAGONAL FORM (PDB 2RAP)
RMSD bond length	0.008
RMSD bond angle	2.065
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	16.000
High resolution limit [Å]	2.200
Rmerge	0.072 *
Total number of observations	144323 *
Number of reflections	16388
<I/σ(I)>	15
Completeness [%]	97.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		PEG 8000 20-25% TRIS-HCL 100MM PH 8 100MM MGCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	20-25 (%)
3	1	reservoir	Tris-HCl	100 (mM)
4	1	reservoir		100 (mM)	or MgCl2