3RA7
Bispecific digoxigenin binding antibodies for targeted payload delivery
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 138.013, 138.013, 123.617 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.040 - 2.798 |
R-factor | 0.2054 |
Rwork | 0.204 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cfd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.292 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.970 |
High resolution limit [Å] | 2.798 | 2.800 |
Number of reflections | 30038 | |
Completeness [%] | 70.5 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 60%(v/v) 2-methyl-1,3-propandiol(MPD), 0.1M sodium acetate, 5mM CaCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |