3QXL
Crystal structure of the CDC25 Domain from Ral-specific Guanine-nucleotide Exchange Factor RalGPS1a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.504, 102.035, 82.141 |
Unit cell angles | 90.00, 96.54, 90.00 |
Refinement procedure
Resolution | 40.800 - 2.237 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.800 |
High resolution limit [Å] | 2.237 |
Number of reflections | 28948 |
Completeness [%] | 74.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 200 mM KCl, 10 mM MgCl2, 50 mM sodium cacodylate and 8.5% (w/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |