3QV0

Crystal structure of Saccharomyces cerevisiae Mam33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-26
Detector	RAYONIX MX-225
Wavelength(s)	0.91940
Spacegroup name	I 21 3
Unit cell lengths	118.755, 118.755, 118.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.22866
Rwork	0.227
R-free	0.25219
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p32
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.970	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.393
Number of reflections	16404
<I/σ(I)>	37.7	10.3
Completeness [%]	99.9	100
Redundancy	22	21.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1 M HEPES, 20% Polyethylene glycol 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K