3QNR
DyPB from Rhodococcus jostii RHA1, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 132.379, 132.379, 160.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.410 - 2.250 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.347 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.073 | 0.577 |
| Number of reflections | 77338 | |
| <I/σ(I)> | 14.2 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5 M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
