3QJG
Epidermin biosynthesis protein EpiD from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.991, 111.256, 154.569 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 45.800 - 2.040 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5q |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.629 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.800 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.040 | 5.560 | 2.040 |
| Rmerge | 0.116 | 0.067 | 0.481 |
| Number of reflections | 162302 | ||
| <I/σ(I)> | 8.1 | 2.08 | |
| Completeness [%] | 95.3 | 99.7 | 74.4 |
| Redundancy | 3.9 | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M Bis-Tris, 20% PEG-MME-5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
