3QC5
GspB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Detector technology | CCD |
Collection date | 2009-06-19 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.681, 96.753, 100.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.700 - 1.400 |
R-factor | 0.144 |
Rwork | 0.142 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.378 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 60816 | |
Completeness [%] | 92.7 | 54.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 296.15 | 6 mg/mL GspB buffered in 20 mM Tris, 33% Jeffamine ED-2001, 0.1 M HEPES, 0.15 M KCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |