3Q6S
The crystal structure of the heterochromatin protein 1 beta chromoshadow domain complexed with a peptide from Shugoshin 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789112 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.816, 108.893, 41.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.408 - 1.930 |
R-factor | 0.1922 |
Rwork | 0.190 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.318 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.800 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 26940 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.6 | 293 | 0.1 M Bis-tris, 0.2 M NaCl, 21% (w/v) PEG 3350, pH 6.6, VAPOR DIFFUSION, temperature 293K |