3Q4H
Crystal structure of the Mycobacterium smegmatis EsxGH complex (MSMEG_0620-MSMEG_0621)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 63 |
Unit cell lengths | 105.598, 105.598, 71.328 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.000 - 2.700 |
R-factor | 0.21022 |
Rwork | 0.207 |
R-free | 0.26790 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.874 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 46063 | |
<I/σ(I)> | 8 | 2.6 |
Completeness [%] | 98.9 | 99.4 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 460mM K-Na-Tartrate, 35% Glycerol, 95mM HEPES, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |