3PQE

Crystal structure of L-lactate dehydrogenase from Bacillus subtilis with H171C mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	133.407, 133.407, 99.335
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.926 - 2.200
R-factor	0.2018
Rwork	0.201
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.926
High resolution limit [Å]	2.200
Number of reflections	99836
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 4000, 0.1M Na/K PO4, PH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K