3PND
FAD binding by ApbE protein from Salmonella enterica: a new class of FAD binding proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 75 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-11 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.103, 120.850, 212.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.600 - 2.750 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o18 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.899 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.600 | 32.599 | 2.820 |
High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
Rmerge | 0.093 | 0.033 | 0.400 |
Total number of observations | 2009 | 13860 | |
Number of reflections | 44268 | ||
<I/σ(I)> | 14.7 | 12 | 1.9 |
Completeness [%] | 99.7 | 91.6 | 99.9 |
Redundancy | 4.2 | 3.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 0.5M LiSO4, 18% polyethylene glycol (PEG) 4000 in 100 mM Tris-HCl, and 10-20 mg/ml as purified FAD bound ApbE, pH 8.5, VAPOR DIFFUSION, temperature 277K |