3P8W
Crystal Structure Analysis of R29M/E81M double mutant of human CLIC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2009-10-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.386, 64.610, 82.984 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.980 - 2.000 |
R-factor | 0.2141 |
Rwork | 0.210 |
R-free | 0.28150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o3t |
RMSD bond length | 0.019 |
RMSD bond angle | 1.758 |
Data reduction software | SAINT |
Data scaling software | SAINT (V7.34A) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.980 | 1.847 | |
High resolution limit [Å] | 1.783 | 3.841 | 1.783 |
<I/σ(I)> | 12.41 | 3.97 | |
Completeness [%] | 98.2 | ||
Redundancy | 7.44 | 4.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 25% (w/v) PEG 2000 monomethyl ether, 0.02% azide, 1 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |