3P40
Crystal structure of neurofascin adhesion complex in space group p3221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.54981 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.508, 94.508, 126.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.920 - 3.200 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.31600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.099 | 0.515 |
| Number of reflections | 12195 | |
| <I/σ(I)> | 13.7 | 4.4 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30%(w/v) PEG400, 0.1 M HEPES, 0.2 lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
