3OM7

Crystal structure of B. megaterium levansucrase mutant Y247W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-24
Detector	MX-225 (Rayonics LLC)
Wavelength(s)	0.91841
Spacegroup name	P 1 21 1
Unit cell lengths	93.527, 100.052, 95.450
Unit cell angles	90.00, 90.51, 90.00

Refinement procedure

Resolution	50.010 - 1.860
R-factor	0.2014
Rwork	0.201
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.000
High resolution limit [Å]	1.860	1.860
Number of reflections	133091
Completeness [%]	98.1	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.1	298	USING 3 uL OF A 8 MG/ML PROTEIN SOLUTION MIXED WITH 3 uL RESERVOIR BUFFER (0.1 M Na-Phosphate-citrate pH 4.1; 0.2 M lithiumsulfate; 20% (w/v) PEG 1000) IN THE DROPLET. , VAPOR DIFFUSION, HANGING DROP, temperature 298K