3OBP

Anaerobic complex of urate oxidase with uric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	291
Detector technology	CCD
Collection date	2008-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	79.853, 96.290, 105.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.2277
Rwork	0.220
R-free	0.24920
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2icq
RMSD bond length	0.030
RMSD bond angle	0.076
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.700	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.068	0.232
Number of reflections	64921
<I/σ(I)>	18.1	3.9
Completeness [%]	99.4	86.17
Redundancy	4.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	liquide diffusion in gel phase	10.5	290	SOLUTION A (0.1 ML): 50MM TRIS BUFFER, SATURATED WITH ACID URIC, 10% PEG 8000, PH 10.5, 0.5 MM AGAROSE IN CAPPILARY (HALF FILLING), SET AS A GEL (BY COOLING FROM 323 K TO ROOM TEMP). SOLUTION B (0.1 ML): 20 MG/ML PROTEIN, SAME BUFFER, SET IN CONTACT WITHIN THE CAPILLARY WITH SOL.A. CRYSTALS DEVELOP IN THE GEL PHASE BY SLOW DIFFUSION. ALL SOLUTION DEGASED, CRYSTALLIZATIONS UNDER ARGON ATMOSPHERE (ANAEROBIC CONDITIONS), liquide diffusion in gel phase, temperature 290K