3O6L
Anti-Tat HIV 11H6H1 Fab' complexed with a 15-mer Tat peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97941 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.760, 69.030, 62.530 |
Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.100 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.22723 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mlb |
RMSD bond length | 0.023 |
RMSD bond angle | 2.047 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.048 | 0.296 |
Number of reflections | 28645 | |
<I/σ(I)> | 19.14 | 4.73 |
Completeness [%] | 99.3 | 99.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | soaking | 6.5 | 289 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% (v/v) polyethylene glycol 550 monomethyl ether, Soaking of Fab' crystals with 0.1mM Tat-peptide for 1 hour, then 0.5mM for 2 hours, soaking, temperature 289K |