3O5L
Fk1 domain mutant A19T of FKBP51, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9723 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 47.978, 47.978, 88.998 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o5p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.554 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.559 | 30.373 | 1.370 |
| High resolution limit [Å] | 1.300 | 4.110 | 1.300 |
| Rmerge | 0.063 | 0.041 | 0.399 |
| Total number of observations | 3651 | 16038 | |
| Number of reflections | 29538 | ||
| <I/σ(I)> | 13.1 | 13.5 | 1.8 |
| Completeness [%] | 99.1 | 89 | 98.2 |
| Redundancy | 4.1 | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 30 % Jeffamine ED-2001, 0.1 M HEPES, pH 7.0, vapor diffusion, temperature 293K |
