3O59
DNA polymerase D large subunit DP2(1-300) from Pyrococcus horikoshii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-23 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.9788, 0.9793, 0.9900 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 54.042, 54.042, 173.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.330 - 2.200 |
R-factor | 0.21246 |
Rwork | 0.206 |
R-free | 0.27168 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.774 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.058 | 0.233 |
Number of reflections | 13924 | |
<I/σ(I)> | 77.9 | 14.4 |
Completeness [%] | 99.0 | 92.4 |
Redundancy | 27.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22.5% PEG 10000, 0.1M HEPES-NaOH, 40mM guanidine-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |