3NJC

Crystal structure of the yslB protein from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR460.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	49.827, 52.105, 136.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.207 - 1.693
R-factor	0.1875
Rwork	0.185
R-free	0.22840
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	500.000	500.000	1.760
High resolution limit [Å]	1.693	3.660	1.700
Rmerge	0.060	0.045	0.369
Rmeas	0.060
Total number of observations	495994
Number of reflections	71912
<I/σ(I)>	15.7
Completeness [%]	94.1	90.8	91.5
Redundancy	6.9	9.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	18-20% PEG 4000, 0.1M HEPES, pH7.0, hanging drop at 291K, VAPOR DIFFUSION, HANGING DROP