3NJ7
1.9 A resolution X-ray structure of (GGCAGCAGCC)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81230 |
Spacegroup name | P 32 |
Unit cell lengths | 47.366, 47.366, 83.209 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.553 - 1.904 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nj6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.372 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.600 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 16346 | |
<I/σ(I)> | 18.2 | 6.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | MgSO4, (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |