3NDQ

Structure of Human TFIIS Domain II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-01
Detector	RAYONIX MX-225
Wavelength(s)	0.97947
Spacegroup name	I 2 2 2
Unit cell lengths	59.270, 60.750, 61.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.693 - 1.933
R-factor	0.168
Rwork	0.166
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dme
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.385	21.690	2.040
High resolution limit [Å]	1.933	6.110	1.933
Rmerge	0.079	0.061	0.110
Total number of observations		2508	17758
Number of reflections	8564
<I/σ(I)>	27	10.6	5
Completeness [%]	99.0	74.2	100
Redundancy	14.2	11	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.0 M Ammonium sulphate, 0.1 M 2-(N-morpholino)ethanesulfonic acid , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K