3NAO
A DNA Crystal Designed to Contain Two Molecules per Asymmetric Unit Cell
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Wavelength(s) | 1.1 |
Spacegroup name | H 3 |
Unit cell lengths | 105.345, 105.345, 183.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.118 - 5.030 |
R-factor | 0.1949 |
Rwork | 0.191 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.260 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.120 |
High resolution limit [Å] | 5.030 |
Number of reflections | 3129 |
Completeness [%] | 85.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SLOW COOLING | 7 | 293 | 125 mM magnesium acetate, 50 mM HEPES (pH 7.0) and 10% MPD in a 100 L sitting drop, equilibrated against a 0.5 ml reservoir of 1.4 M ammonium sulphate for two weeks. SLOW COOLING, temperature changed from 363K to 293K. |
1 | SLOW COOLING | 7 | 293 | 125 mM magnesium acetate, 50 mM HEPES (pH 7.0) and 10% MPD in a 100 L sitting drop, equilibrated against a 0.5 ml reservoir of 1.4 M ammonium sulphate for two weeks. SLOW COOLING, temperature changed from 363K to 293K. |