3NA7
2.2 Angstrom Structure of the HP0958 Protein from Helicobacter pylori CCUG 17874
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-07-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.984 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.720, 37.951, 66.056 |
Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.25067 |
Rwork | 0.248 |
R-free | 0.29378 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.316 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.220 |
Number of reflections | 19056 | |
<I/σ(I)> | 33.5 | 3.5 |
Completeness [%] | 91.6 | 65.7 |
Redundancy | 5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG 3350, 0.1M HEPES pH 7.5, 0.2M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |