3MKO
Crystal Structure of the Lymphocytic Choriomeningitis Virus Membrane Fusion Glycoprotein GP2 in its Postfusion Conformation
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-08-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.3354 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 52.925, 52.925, 191.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.220 - 1.800 |
Rwork | 0.188 |
R-free | 0.22220 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.220 | 1.940 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.061 | 0.463 |
Number of reflections | 13777 | |
<I/σ(I)> | 26.9 | 2.5 |
Completeness [%] | 89.9 | 88.2 |
Redundancy | 13.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% MPD, 2% PEG 400, 100mM imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |