3MJB
Cricket Paralysis Virus IGR IRES Domain 3 RNA bound to sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Wavelength(s) | 1.1271 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.940, 58.940, 99.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 2.800 |
Rwork | 0.242 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b31 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.388 |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.680 |
High resolution limit [Å] | 2.200 |
Number of reflections | 9451 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 1.4 M lithium sulfate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH; transferred to saturated lithium sulfate, 42.5 mM magnesium chloride, 0.5 mM spermidine, 10 mM HEPES-KOH, 50 mM HEPES-NaOH for cryo-protection, pH 7.5, VAPOR DIFFUSION, temperature 293K |