3MDW
The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 304.046, 67.247, 98.229 |
Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
Resolution | 39.427 - 1.898 |
R-factor | 0.1714 |
Rwork | 0.170 |
R-free | 0.20350 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.042 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.430 |
High resolution limit [Å] | 1.898 |
Rmerge | 0.073 |
Number of reflections | 148848 |
Completeness [%] | 94.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 1.4M Sodium Phosphate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293.0 |