3M15
A Zn-mediated asymmetric trimer of a cytochrome cb562 variant (D74A-RIDC1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.879, 74.819, 81.358 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.900 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.220 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.524 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 9996 | |
| <I/σ(I)> | 7.3 | 1.97 |
| Completeness [%] | 99.8 | 81.1 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
