3LUC

Crystal structure of MID domain from hAGO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9785
Spacegroup name	P 1
Unit cell lengths	39.986, 46.766, 66.437
Unit cell angles	85.69, 72.20, 83.86

Refinement procedure

Resolution	30.000 - 1.691
R-factor	0.1758
Rwork	0.174
R-free	0.20950
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.596	1.751
High resolution limit [Å]	1.691	1.691
Number of reflections	46563
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M imidazole, 0.2 M NaCl, 0.46 M NaH2PO4, 1.84 M K2HPO4. protein: 15 mg/mL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K