3LSG
The crystal structure of the C-terminal domain of the two-component response regulator yesN from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97901 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.224, 66.224, 245.401 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.290 - 2.049 |
R-factor | 0.1875 |
Rwork | 0.185 |
R-free | 0.24040 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.944 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.300 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.106 | 0.763 |
Number of reflections | 39683 | |
<I/σ(I)> | 26.2 | 2.6 |
Completeness [%] | 98.0 | 95.3 |
Redundancy | 8.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 0.2M di-sodium hydrogen phosphate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |