3LL2
Monomeric Griffithsin in Complex with a High-Mannose Branched Carbohydrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 |
Unit cell lengths | 90.500, 90.500, 27.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 0.970 |
R-factor | 0.144 |
Rwork | 0.143 |
R-free | 0.15500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lky |
RMSD bond length | 0.020 |
RMSD bond angle | 2.579 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.3) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.000 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.055 | 0.507 |
Number of reflections | 66935 | |
<I/σ(I)> | 14.8 | 2 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 4.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M mixture of imidazole, sodium cacodylate, MES, and bis-tris, 0.1 M mixture of L-Na-glutamate, alanine, glycine, lysine-HCl, and serine, 30% w/v PEG MME 550 and PEG 20000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |