3L88
Crystal structure of the human Adenovirus type 21 fiber knob
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-08-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 85.000, 63.920, 209.350 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 49.450 - 2.500 |
| R-factor | 0.23543 |
| Rwork | 0.234 |
| R-free | 0.25905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7z |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.594 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 2.680 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.203 |
| Number of reflections | 71876 | |
| <I/σ(I)> | 8.83 | 4.2 |
| Completeness [%] | 91.6 | 78.8 |
| Redundancy | 3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 298 | 22% PEG 3000, 0.2 M NaCl, 0.1 M Tris, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
