3L2X
Crystal Structure of Spin Labeled T4 Lysozyme Mutant 115-119RX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-03 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.113, 60.113, 96.655 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.419 - 1.800 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c6t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.116 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.000 | 80.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Number of reflections | 18829 | ||
<I/σ(I)> | 12.3 | ||
Completeness [%] | 97.4 | 99.8 | 80.9 |
Redundancy | 9.3 | 9.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | 2.0M dibasic potassium phosphate and monobasic sodium phosphate, 0.25M sodium chloride, 0.04% sodium azide, saturated with bis(2-hydroxyethyl) disulfide, pH 6.4, vapor diffusion, hanging drop, temperature 277K |