3L2U
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and GS9137 (Elvitegravir)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2009-10-13 |
| Detector | ADSC |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 157.100, 157.100, 124.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.440 - 3.150 |
| R-factor | 0.19416 |
| Rwork | 0.193 |
| R-free | 0.21836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dlr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.551 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.102 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 27284 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50mM Mes-NaOH, pH 6.5, vapor diffusion, hanging drop, temperature 291K |
