Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 95 |
| Detector technology | CCD detector |
| Collection date | 2004-12-04 |
| Detector | Dual Quantum 4 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 128.039, 128.039, 128.039 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.270 - 2.200 |
| Rwork | 0.218 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1akm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.460 |
| Number of reflections | 17844 | |
| <I/σ(I)> | 28.5 | 4.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6 | 291 | Lithium sulfate, Bis-tris, PEG3350, pH 6.0, hanging drop, temperature 291K |
