3KSK
Crystal Structure of single chain PvuII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81280 |
Spacegroup name | P 42 |
Unit cell lengths | 101.921, 101.921, 100.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 102.060 - 2.350 |
R-factor | 0.219 |
Rwork | 0.215 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 2.087 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.060 | 2.411 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 42525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 1.28-1.36 M ammonium sulfate, 5% MPD, 100 mM bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |