3KRN
Crystal Structure of C. elegans cell-death-related nuclease 5(CRN-5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 102 |
Detector technology | CCD |
Collection date | 2008-11-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.954, 44.837, 65.507 |
Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
Resolution | 26.505 - 3.918 |
R-factor | 0.3101 |
Rwork | 0.306 |
R-free | 0.35640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nn6 chain D |
RMSD bond length | 0.005 |
RMSD bond angle | 0.989 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.040 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.071 | 0.197 |
Total number of observations | 9991 | |
Number of reflections | 3235 | |
<I/σ(I)> | 13.8 | 5.25 |
Completeness [%] | 97.7 | 91.3 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl, 0.2M NaCl, 25% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |