3K58
Crystal structure of E.coli Pol II-normal DNA-dTTP ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.823, 100.296, 126.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.050 |
Rwork | 0.198 |
R-free | 0.20300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3k57 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.604 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.073 | 0.670 |
Number of reflections | 62987 | |
<I/σ(I)> | 17.6 | 2.3 |
Completeness [%] | 96.8 | 96.9 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 20% PEGmme5000, 0.2 M ammonium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |