3JYZ
Crystal structure of Pseudomonas aeruginosa (strain: Pa110594) typeIV pilin in space group P41212
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.059, 63.059, 67.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.842 - 1.550 |
R-factor | 0.1801 |
Rwork | 0.177 |
R-free | 0.20880 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.550 |
Rmerge | 0.068 |
Number of reflections | 20404 |
<I/σ(I)> | 33.9 |
Completeness [%] | 100.0 |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.1 M Tris-HCl pH 8.5, 2.0 M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |