3JW1
Crystal Structure of Bovine Pancreatic Ribonuclease Complexed with Uridine-5'-monophosphate at 1.60 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-29 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.730, 74.850, 50.520 |
Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
Resolution | 29.540 - 1.600 |
R-factor | 0.203 |
Rwork | 0.197 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.549 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.027 | 0.340 |
Number of reflections | 25900 | |
<I/σ(I)> | 16.8 | 2.4 |
Completeness [%] | 91.2 | 64.2 |
Redundancy | 2.61 | 2.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 0.1 M HEPES BUFFER; Reservoir of 25% PEG3350 in water; droplets were 5-10 mM in basic fuchsin, tobramycin, uridine-5'-monophosphate and 1 mM in ribonuclease A, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |