3JTT
Cystal structure of Rhesus macaque MHC class I:Mamu-A*02
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5478 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 128.993, 129.007, 129.026 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.293 - 2.800 |
R-factor | 0.2158 |
Rwork | 0.214 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zvs |
RMSD bond length | 0.002 |
RMSD bond angle | 0.660 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.074 | 0.528 |
Number of reflections | 51015 | |
<I/σ(I)> | 37.2 | 4.7 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M Bis-Tris pH 5.5, 2 M ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 291K |