3JSA
Homoserine dehydrogenase from Thermoplasma volcanium complexed with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 110.043, 110.043, 60.453 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.700 - 1.950 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.608 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.700 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.839 |
| Number of reflections | 30935 | |
| <I/σ(I)> | 21.3 | 2.42 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M sodium chloride, 0.1 M sodium cacodylate, 2 M ammonium sulfate, NAD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
