3JPY
Crystal structure of the zinc-bound amino terminal domain of the NMDA receptor subunit NR2B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2826 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 143.368, 143.368, 88.467 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.290 - 3.209 |
R-factor | 0.2056 |
Rwork | 0.204 |
R-free | 0.23290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jpw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.041 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.078 | 0.590 |
Number of reflections | 17197 | |
<I/σ(I)> | 16.8 | 3.1 |
Completeness [%] | 98.4 | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 3.3M NaCl, 2% PEG400, 0.1M MgCl2, 0.1M acetate, 0.1mM ZnCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |