3JC5
Structure of the eukaryotic replicative CMG helicase and pumpjack motion
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for 3 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
| Number of Images | 8000 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.7 |
| Software | UCSF Chimera,RELION |
| Symmetry | C1 |
