EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 1503 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.5 |
| Software | Coot,REFMAC,SPDBV,FREALIGN |
| Symmetry | I |
