3ISM
Crystal structure of the EndoG/EndoGI complex: Mechanism of EndoG inhibition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.790, 109.150, 121.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.230 - 2.200 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o3b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.230 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 46364 | |
| <I/σ(I)> | 14.8 | 4.2 |
| Completeness [%] | 95.9 | 90.5 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20%(w/v)PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
