3IOW
Huntingtin amino-terminal region with 17 Gln residues - crystal C99-Hg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9800 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.097, 162.770, 139.660 |
Unit cell angles | 90.00, 98.85, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.500 |
R-factor | 0.24511 |
Rwork | 0.243 |
R-free | 0.28095 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ior |
RMSD bond length | 0.007 |
RMSD bond angle | 0.962 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 32995 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 12% PEG 4000, 200 mM Zn acetate, 200 mM Sodium acetate, 100 mM Sodium Cacodylate pH 6.5-7.4, VAPOR DIFFUSION, HANGING DROP, temperature 278K |