3I9Z
Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.3800 |
| Spacegroup name | P 63 |
| Unit cell lengths | 63.960, 63.960, 27.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.1908 |
| Rwork | 0.191 |
| R-free | 0.26490 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.238 |
| Number of reflections | 5019 | |
| <I/σ(I)> | 29.1 | 8.6 |
| Completeness [%] | 99.9 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30 % w/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
