3HXK
Crystal Structure of a sugar hydrolase (YeeB) from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR108
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97907 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.391, 150.425, 218.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 3.200 |
R-factor | 0.257 |
Rwork | 0.256 |
R-free | 0.29600 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.088 | 0.172 |
Number of reflections | 36064 | |
<I/σ(I)> | 17.4 | 3.2 |
Completeness [%] | 92.6 | 74.5 |
Redundancy | 5.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch, under oil | 9.1 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1 M TRIS (pH 9.1), 29% PEG400, and 0.1M (Mg)2SO4. , microbatch, under oil, temperature 291K |