3HX3
Crystal structure of CRALBP mutant R234W
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-09-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.930, 57.880, 75.150 |
| Unit cell angles | 90.00, 122.85, 90.00 |
Refinement procedure
| Resolution | 45.577 - 1.690 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.18400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.176 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.577 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.048 | 0.576 |
| Number of reflections | 67824 | 9663 |
| <I/σ(I)> | 19.8 | 2.7 |
| Completeness [%] | 97.4 | 86.1 |
| Redundancy | 5.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 3000, NaCl, pH 7.5, VAPOR DIFFUSION, temperature 298K |
