3HVO
Structure of the genotype 2B HCV polymerase bound to a NNI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-23 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.293, 64.648, 135.714 |
Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
Resolution | 19.840 - 2.000 |
R-factor | 0.18353 |
Rwork | 0.182 |
R-free | 0.21823 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gsz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.208 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 88994 | |
<I/σ(I)> | 10.5 | 2.2 |
Completeness [%] | 98.0 | 93.7 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 300 | 10% PEG4000, 0.1M sodium citrate, 0.2M sodium chloride, 5mM DTT, Protein concentration 6.5mg/ml, pH6.1, VAPOR DIFFUSION, SITTING DROP, temperature 300K |